Rapid Effective Trace-Back Capability Value in Reducing the Cost of a Foot and Mouth Disease Event

This study evaluates how the availability of animal tracing affects the cost of a hypothetical Foot and Mouth Disease (FMD) outbreak in the Texas High Plains using alternative tracing scenarios. To accomplish this objective, the AusSpread epidemic disease spread model (Ward et al., 2006) is used to simulate a High Plains FMD outbreak under different animal tracing possibilities. A simple economic costing module (Elbakidze, 2008) is used to determine the savings in terms of animal disease mitigation costs from rapid, effective trace-back. The savings from increased traceability are then be compared to the cost of a functional National Animal Identification System (NAIS). Initial results indicate that rapid, effective tracing reduces the overall cost of disease outbreaks and that the benefits per animal in terms of reduced cost of an outbreak more than outweigh the annualized cost per animal of implementing a NAIS. A value of time related to controlling an outbreak is estimated to have increased benefits from an identification system that incorporates a rapid response capability. We also find the level of benefits vary depending on the location of initial infection and whether or not welfare slaughter occurs.Traceability, Foot and Mouth Disease, Economics, Agricultural and Food Policy, Livestock Production/Industries,

Modeling Thermoregulatory Responses to Cold Environments

The ability to model and simulate the rise and fall of core body temperature is of significant interest to a broad spectrum of organizations. These organizations include the military, as well as both public and private health and medical groups. To effectively use cold models, it is useful to understand the first principles of heat transfer within a given environment as well as have an understanding of the underlying physiology, including the thermoregulatory responses to various conditions and activities. The combination of both rational or first principles and empirical approaches to modeling allow for the development of practical models that can predict and simulate core body temperature changes for a given individual and ultimately provide protection from injury or death. The ability to predict these maximal potentials within complex and extreme environments is difficult. The present work outlines biomedical modeling techniques to simulate and predict cold-related injuries, and discusses current and legacy models and methods

Confirmation of a recent bipolar ejection in the very young hierarchical multiple system IRAS 16293-2422

We present and analyze two new high-resolution (approx 0.3 arcsec), high-sensitivity (approx 50 uJy beam-1) Very Large Array 3.6 cm observations of IRAS 16293-2422 obtained in 2007 August and 2008 December. The components A2alpha and A2beta recently detected in this system are still present, and have moved roughly symmetrically away from source A2 at a projected velocity of 30-80 km s-1. This confirms that A2alpha and A2beta were formed as a consequence of a very recent bipolar ejection from A2. Powerful bipolar ejections have long been known to occur in low-mass young stars, but this is -to our knowledge-- the first time that such a dramatic one is observed from its very beginning. Under the reasonable assumption that the flux detected at radio wavelengths is optically thin free-free emission, one can estimate the mass of each ejecta to be of the order of 10^-8 Msun. If the ejecta were created as a consequence of an episode of enhanced mass loss accompanied by an increase in accretion onto the protostar, then the total luminosity of IRAS 16293-2422 ought to have increased by 10-60% over the course of at least several months. Between A2alpha and A2beta, component A2 has reappeared, and the relative position angle between A2 and A1 is found to have increased significantly since 2003-2005. This strongly suggests that A1 is a protostar rather than a shock feature, and that the A1/A2 pair is a tight binary system. Including component B, IRAS 16293-2422 therefore appears to be a very young hierarchical multiple system.Comment: Accepted for publication in The Astrophysical Journa

Mission Concept for the Single Aperture Far-Infrared (SAFIR) Observatory

The Single Aperture Far-InfraRed (SAFIR) Observatory's science goals are driven by the fact that the earliest stages of almost all phenomena in the universe are shrouded in absorption by and emission from cool dust and gas that emits strongly in the far-infrared and submillimeter. Over the past several years, there has been an increasing recognition of the critical importance of this spectral region to addressing fundamental astrophysical problems, ranging from cosmological questions to understanding how our own Solar System came into being. The development of large, far-infrared telescopes in space has become more feasible with the combination of developments for the James Webb Space Telescope and of enabling breakthroughs in detector technology. We have developed a preliminary but comprehensive mission concept for SAFIR, as a 10 m-class far-infrared and submillimeter observatory that would begin development later in this decade to meet the needs outlined above. Its operating temperature (<4K) and instrument complement would be optimized to reach the natural sky confusion limit in the far-infrared with diffraction-limited peformance down to at least 40 microns. This would provide a point source sensitivity improvement of several orders of magnitude over that of Spitzer or Herschel, with finer angular resolution, enabling imaging and spectroscopic studies of individual galaxies in the early universe. We have considered many aspects of the SAFIR mission, including the telescope technology, detector needs and technologies, cooling method and required technology developments, attitude and pointing, power systems, launch vehicle, and mission operations. The most challenging requirements for this mission are operating temperature and aperture size of the telescope, and the development of detector arrays.Comment: 36 page

Water vapor in the atmosphere: an examination for CARMA phase correction

We report on the current progress of the water vapor radiometer (WVR) phase correction project for the Combined Array for Research in Millimeter-wave Astronomy (CARMA). CARMA is a new millimeter array that merges the Owens Valley Radio Observatory (OVRO) array, the Berkeley-Illinois-Maryland Association (BIMA) array and eventually the Sunyaev-Zel'dovich Array (SZA). WVRs are designed for phase correction by monitoring the water vapor in the atmosphere along the line of sight toward astronomical sources. In addition, we discuss the stability of the current OVRO water vapor radiometers in preparation for testing at the CARMA site. We will systematically analyze the receivers with atmospheric correlations to decouple the effects of instrumentation and atmospheric noise. Finally, we report on the status of the correlation receivers in development

Relationships of Physical Performance Tests to Military-relevant Tasks in Women

Purpose: This investigation sought to determine the most predictive measures of performance on a repetitive box lifting task (RBLT) and load bearing task (LBT) among 123 women (aged 23±4 years, height 165±7 cm, body mass 64±10 kg). Methods: To determine the relationship of various predictors to performance on the RBLT and LBT, multiple regression analysis was conducted on body mass, height, leg cross-sectional area, upper and lower body muscular strength, lower body explosive power, upper and lower body local muscular endurance, and aerobic capacity. Results: The mean±SD (range) number of repetitions for the RBLT was 86±23 (20-159). The mean±SD (range) time to complete the LBT was 2,054±340 seconds (1,307-3,447). The following equations were generated: RBLT (number of repetitions)=57.4 + 0.2(peak jump power) + 0.4(number of pushups in 2 minutes) + 0.15(number of repetitions during the squat endurance test) + 1.39(one repetition maximal strength boxlift (kg)) – 0.04(2-mile run time (2MR) in seconds), R=0.81; standard error of the estimate (SEE)=14; LBT (in seconds)=1,831 – 4.28(number of repetitions during the squat endurance test) + 0.95(2MR in seconds) – 13.4(body mass), R=0.73; SEE=232. Conclusions: We found that the 2MR and squat endurance test were signifi cant predictive factors for performance on both load carriage tasks. These data also imply that women’s performance in combat-related tasks can be improved with training that targets muscular strength, power, and local muscular endurance in addition to aerobic capacity

A Spatially Resolved Inner Hole in the Disk around GM Aurigae

We present 0.3 arcsec resolution observations of the disk around GM Aurigae with the Submillimeter Array (SMA) at a wavelength of 860 um and with the Plateau de Bure Interferometer at a wavelength of 1.3 mm. These observations probe the distribution of disk material on spatial scales commensurate with the size of the inner hole predicted by models of the spectral energy distribution. The data clearly indicate a sharp decrease in millimeter optical depth at the disk center, consistent with a deficit of material at distances less than ~20 AU from the star. We refine the accretion disk model of Calvet et al. (2005) based on the unresolved spectral energy distribution (SED) and demonstrate that it reproduces well the spatially resolved millimeter continuum data at both available wavelengths. We also present complementary SMA observations of CO J=3-2 and J=2-1 emission from the disk at 2" resolution. The observed CO morphology is consistent with the continuum model prediction, with two significant deviations: (1) the emission displays a larger CO J=3-2/J=2-1 line ratio than predicted, which may indicate additional heating of gas in the upper disk layers; and (2) the position angle of the kinematic rotation pattern differs by 11 +/- 2 degrees from that measured at smaller scales from the dust continuum, which may indicate the presence of a warp. We note that photoevaporation, grain growth, and binarity are unlikely mechanisms for inducing the observed sharp decrease in opacity or surface density at the disk center. The inner hole plausibly results from the dynamical influence of a planet on the disk material. Warping induced by a planet could also potentially explain the difference in position angle between the continuum and CO data sets.Comment: 12 pages, 6 figures, accepted for publication in Ap

Activity of the DNA minor groove cross-linking agent SG2000 (SJG-136) against canine tumours

BACKGROUND: Cancer is the leading cause of death in older dogs and its prevalence is increasing. There is clearly a need to develop more effective anti-cancer drugs in dogs. SG2000 (SJG-136) is a sequence selective DNA minor groove cross-linking agent. Based on its in vitro potency, the spectrum of in vivo and clinical activity against human tumours, and its tolerability in human patients, SG2000 has potential as a novel therapeutic against spontaneously occurring canine malignancies. RESULTS: In vitro cytotoxicity was assessed using SRB and MTT assays, and in vivo activity was assessed using canine tumour xenografts. DNA interstrand cross-linking (ICL) was determined using a modification of the single cell gel electrophoresis (comet) assay. Effects on cell cycle distribution were assessed by flow cytometry and measurement of γ-H2AX by immunofluorescence and immunohistochemistry. SG2000 had a multi-log differential cytotoxic profile against a panel of 12 canine tumour cell lines representing a range of common tumour types in dogs. In the CMeC-1 melanoma cell line, DNA ICLs increased linearly with dose following a 1 h treatment. Peak ICL was achieved within 1 h and no removal was observed over 48 h. A relationship between DNA ICL formation and cytotoxicity was observed across cell lines. The formation of γ-H2AX foci was slow, becoming evident after 4 h and reaching a peak at 24 h. SG2000 exhibited significant anti-tumour activity against two canine melanoma tumour models in vivo. Anti-tumour activity was observed at 0.15 and 0.3 mg/kg given i.v. either once, or weekly x 3. Dose-dependent DNA ICL was observed in tumours (and to a lower level in peripheral blood mononuclear cells) at 2 h and persisted at 24 h. ICL increased following the second and third doses in a repeated dose schedule. At 24 h, dose dependent γ-H2AX foci were more numerous than at 2 h, and greater in tumours than in peripheral blood mononuclear cells. SG2000-induced H2AX phosphorylation measured by immunohistochemistry showed good correspondence, but less sensitivity, than measurement of foci. CONCLUSIONS: SG2000 displayed potent activity in vitro against canine cancer cell lines as a result of the formation and persistence of DNA ICLs. SG2000 also had significant in vivo antitumour activity against canine melanoma xenografts, and the comet and γ-H2AX foci methods were relevant pharmacodynamic assays. The clinical testing of SG2000 against spontaneous canine cancer is warranted. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12917-015-0534-2) contains supplementary material, which is available to authorized users

Drone-based Water Sampling and Characterization of Three Freshwater Harmful Algal Blooms in the United States

Freshwater harmful algal blooms (HABs), caused mostly by toxic cyanobacteria, produce a range of cyanotoxins that threaten the health of humans and domestic animals. Climate conditions and anthropogenic influences such as agricultural run-off can alter the onset and intensity of HABs. Little is known about the distribution and spread of freshwater HABs. Current sampling protocols in some lakes involve teams of researchers that collect samples by hand from a boat and/or from the shoreline. Water samples can be collected from the surface, from discrete-depth collections, and/or from depth-integrated intervals. These collections are often restricted to certain months of the year, and generally are only performed at a limited number of collection sites. In lakes with active HABs, surface samples are generally sufficient for HAB water quality assessments. We used a unique DrOne Water Sampling SystEm (DOWSE) to collect water samples from the surface of three different HABs in Ohio (Grand Lake St Marys, GLSM and Lake Erie) and Virginia (Lake Anna), United States in 2019. The DOWSE consisted of a 3D-printed sampling device tethered to a drone (uncrewed aerial system, or UAS), and was used to collect surface water samples at different distances (10–100 m) from the shore or from an anchored boat. One hundred and eighty water samples (40 at GLSM, 20 at Lake Erie, and 120 at Lake Anna) were collected and analyzed from 18 drone flights. Our methods included testing for cyanotoxins, phycocyanin, and nutrients from surface water samples. Mean concentrations of microcystins (MCs) in drone water samples were 15.00, 1.92, and 0.02 ppb for GLSM, Lake Erie, and Lake Anna, respectively. Lake Anna had low levels of anatoxin in nearly all (111/120) of the drone water samples. Mean concentrations of phycocyanin in drone water samples were 687, 38, and 62 ppb for GLSM, Lake Erie, and Lake Anna, respectively. High levels of total phosphorus were observed in the drone water samples from GLSM (mean of 0.34 mg/L) and Lake Erie (mean of 0.12 mg/L). Lake Anna had the highest variability of total phosphorus with concentrations that ranged from 0.01 mg/L to 0.21 mg/L, with a mean of 0.06 mg/L. Nitrate levels varied greatly across sites, inverse with bloom biomass, ranging from below detection to 3.64 mg/L, with highest mean values in Lake Erie followed by GLSM and Lake Anna, respectively. Drones offer a rapid, targeted collection of water samples from virtually anywhere on a lake with an active HAB without the need for a boat which can disturb the surrounding water. Drones are, however, limited in their ability to operate during inclement weather such as rain and heavy winds. Collectively, our results highlight numerous opportunities for drone-based water sampling technologies to track, predict, and respond to HABs in the future

Pre-surgical radiologic identification of peri-prosthetic osteolytic lesions around TKRs: a pre-clinical investigation of diagnostic accuracy

Background: Emerging longitudinal data appear to demonstrate an alarming trend towards an increasing prevalence of osteolysis-induced mechanical failure, following total knee replacement (TKR). Even with high-quality multi-plane X-rays, accurate pre-surgical evaluation of osteolytic lesions is often difficult. This is likely to have an impact on surgical management and provides reasonable indication for the development of a model allowing more reliable lesion assessment. The aim of this study, using a simulated cadaver model, was to explore the accuracy of rapid spiral computed tomography (CT) examination in the non-invasive evaluation of peri-prosthetic osteolytic lesions, secondary to TKR, and to compare this to conventional X-ray standards. Methods: A series of nine volume-occupying defects, simulating osteolytic lesions, were introduced into three human cadaveric knees, adjacent to the TKR implant components. With implants in situ, each knee was imaged using a two-stage conventional plain X-ray series and rapid-acquisition spiral CT. A beam-hardening artefact removal algorithm was employed to improve CT image quality. After random image sorting, 12 radiologists were independently shown the series of plain X-ray images and asked to note the presence, anatomic location and 'size' of osteolytic lesions observed. The same process was repeated separately for review of the CT images. The corresponding X-ray and CT responses were directly compared to elicit any difference in the ability to demonstrate the presence and size of osteolytic lesions. Results: Access to CT images significantly improved the accuracy of recognition of peri-prosthetic osteolytic lesions when compared to AP and lateral projections alone (P = 0.008) and with the addition of bi-planar oblique X-rays (P = 0.03). No advantage was obtained in accuracy of identification of such lesions through the introduction of the oblique images when compared with the AP and lateral projections alone (P = 0.13) Conclusion: The findings of this study suggest that peri-prosthetic osteolytic lesions can be reliably described non-invasively using a simple, rapid-acquisition CT-based imaging approach. The low sensitivity of conventional X-ray, even with provision of supplementary bi-planar 45° oblique views, suggests a limited role for use in situ for TKR implant screening where peri-prosthetic osteolytic lesions are clinically suspected. In contrast, the accuracy of CT evaluation, linked to its procedural ease and widespread availability, may provide a more accurate way of evaluating osteolysis around TKRs, at routine orthopaedic follow up. These findings have direct clinical relevance, as accurate early recognition and classification of such lesions influences the timing and aggressiveness of surgical and non-operative management strategies, and also the nature and appropriateness of planned implant revision or joint-salvaging osteotomy procedures.Timothy P. Kurmis, Andrew P. Kurmis, David G. Campbell and John P. Slavotine